JBrowser

Interactive Genome Visualization for EPS Mutant Analysis

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Maize CA7301 Genome
Zea mays L. | Elite Inbred Line
We sequenced and assembled a high-quality genome of the elite maize inbred line CA7301 using PacBio long-read sequencing technology. The genome was comprehensively annotated, and we performed whole-genome sequencing (WGS) on EPS mutant materials using Illumina sequencing. SNP analysis identified mutation sites across the genome.
60 Individuals Sequenced
30Γ— Sequencing Depth
181,420 Total SNPs
3,425 Non-synonymous SNPs
Mutation Effect Analysis: All genome-wide SNPs were analyzed using EVO2 and PlantCAD2 models. Non-synonymous mutations were subjected to protein structure prediction using ESMFold, followed by mutation effect assessment using ESM-1V, ESM2, ProtSSN, and SaProt models. All data have been integrated into the genome browser.
EVO2 Analysis PlantCAD2 ESMFold Structure ESM-1V/ESM2 ProtSSN SaProt
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🍚
Rice Huanghuazhan Genome
Oryza sativa L. | Indica Variety
The genome data was sourced from published research on long-read sequencing of rice genomes. We performed whole-genome sequencing on EPS mutant progeny and obtained comprehensive SNP data integrated into the genome browser.
Reference: "Long-read sequencing of 111 rice genomes reveals significantly larger pan-genomes" - Genome data derived from this published study.
32 Individuals Sequenced
26,271 Total SNPs
649 Non-synonymous SNPs
Mutation Effect Analysis: All genome-wide SNPs were analyzed using EVO2 and PlantCAD2 models. Non-synonymous mutations were subjected to protein structure prediction using ESMFold, followed by mutation effect assessment using ESM-1V, ESM2, ProtSSN, and SaProt models. All data have been integrated into the genome browser.
PlantCAD2 EVO2 Analysis ESMFold Structure ESM-1V/ESM2 ProtSSN SaProt
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🀝 Collaboration Opportunities
We have a large collection of phenotyped materials that have not been fully characterized, representing excellent opportunities for collaborative research. We welcome partnerships to jointly develop and explore the EPS project potential.
🌽 Maize
Large collection of phenotyped mutant materials with diverse traits ready for collaborative analysis
🍚 Rice Huanghuazhan
Extensive mutant population with phenotypic data available for joint research
🍚 Rice Xiaowei
Multiple sub-lines including 9311 and Nipponbare backgrounds
🌾 Wheat Jimai 38
Hexaploid wheat mutant collection with phenotypic variations
🌾 Wheat Zhongmai 578
Elite wheat variety with established mutant populations
🌾 Wheat Zhongmai 895
High-quality wheat genetic resource for collaborative studies
Interested in collaboration?
Please contact Prof. Chuanxiao Xie to discuss potential research partnerships and material sharing opportunities.